Exon—intron organization of the Arabidopsis thaliana protein kinase genes CDC2a and CDC2b

FEBS Letters

Endoh, Hideki¹  Hirayama, Takashi¹  Imajuku, Yoshiro¹  Oka, Atsuhiro¹ 

[1] Laboratory of Molecular Genetics, Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan

关键词: Cell cycle control;    Evolution;    Gene structure;    Higher plant;    Nucleotide sequence;    bp;    base-pair(s);    cDNA;    DNA complementary to mRNA;    kb;    kilobase(s) or 1000 bp;    nt;    nucleotide position(s);    p34 cdc2;    34-kDa protein encoded by cdc2 or cdc2-like genes;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(92)80592-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown by cDNA cloning that a higher plant, Arabidopsis thaliana, possesses at least two CDC2 genes (CDC2a and CDC2b) similar to the cell-cycle-controlling cdc2 gene of Schizosaccharomyces pombe. To understand the exon—intron organization of these genes, genomic clones were isolated and their nucleotide sequences determined. The coding and 5′-untranslated regions of CDC2a were interrupted by seven and one introns, respectively, whilst CDC2b contained three introns within the coding portion. These intron positions partly overlapped with each other and with those of the yeast cdc2 gene, nevertheless the lengths and sequences of the corresponding introns were diverse.

【 授权许可】

Unknown