Polyamines enhance calcium mobilization in fMet‐Leu‐Phe‐stimulated phagocytes

FEBS Letters

Walters, John D.¹  Chapman, Kena J.¹  Sorboro, David M.¹ 

[1] Department of Periodontology, College of Dentistry, The Ohio State University, Columbus, OH 43210, USA

关键词: Polyamine;    Calcium mobilization;    Protein kinase C;    Chemotactic peptide;    Phagocytic leukocyte;    fMLP;    N-formylmethyionyl-leucylphenylalanine;    fura-2/AM;    fura-2 acetomethoxy ester;    1P3;    inositol 1;    4;    5-trisphosphate;    PDBu;    phorbol 12;    13-dibutyrate;    PKC;    protein kinase C;    PMN;    polymorphonuclear leukocyte;   

DOI  :  10.1016/0014-5793(92)80584-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spermidine and putrescine (50 μM-1 mM), found in exudates from infection sites, significantly enhance fMet-Leu-Phe-induced Ca²⁺ mobilization in differentiated HL-60 cells and polymorphonuclear leukocytes (PMNs) by delaying the return to basal cytosolic Ca²⁺ levels. This enhancement by polyamines is associated with inhibition of Ca²⁺ efflux across the plasma membrane. In parallel with their effects on Ca²⁺ signaling, polyamines also significantly prolong the kinetics of fMet-Leu-Phe-induced protein kinase C translocation. Thus, polyamines may play a novel role in modulating regulatory events in phagocytes.

【 授权许可】

Unknown