Comment to Shinohara et al. (1991) FEBS Letters 293, 1731̄74 The uncoupling protein is not expressed in rat liver

FEBS Letters

Raimbault, Serge¹  Miroux, Bruno¹  Ricquier, Daniel¹  Bouillaud, Frédéric¹  Champigny, Odette¹ 

[1] Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, Centre National de la Recherche Scientifique, 9 rue Jules Hetzel, 92190 Meudon, France

关键词: Uncoupling protein;    Liver mitochondria;    Brown adipose tissue;    Northern blotting;    Immunoblotting;    Polymerase chain reaction;   

DOI  :  10.1016/0014-5793(92)80496-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using Northern blot analysis, immunoblotting with purified antibodies and Polymerase Chain Reaction analysis, we were unable to detect the Uncoupling Protein-UCP or its mRNA in liver of control, cold-exposed or newborn rats. The unique expression of this protein in brown adipocytes was confirmed. These data refute the surprising recent report on UCP expression in rat liver (Shinohara (1991) FEBS Lett. 293, 173–174). Moreover we report that the hybridization signal obtained by these authors is probably non-specific and due to the 3′ non-coding domain of the UCP cDNA probe.

【 授权许可】

Unknown