Effects of 2(3)‐tert‐butyl‐4‐hydroxyanisole (BHA) on in situ mitochondria of Trypanosoma cruzi

FEBS Letters

Morello, A.³  Repetto, Y.³  Aldunate, J.³  Ojeda, J.M.²  Coloma-Torres, L.¹  Spencer, P.³ 

[1] Departinent of Biology, Faculty of Basic Sciences, Metropolitan University of Educational Sciences, Santiago, Chile;Department of Microbiology and Parasitology, Virology Unit, Faculty of Medicine, University of Chile, Santiago, Chile;Department of Biochemistry, Faculty of Medicine, University of Chile, P.O. Box 70086, Santiago, Chile

关键词: Oxygen uptake;    2(3)-tert-Butyl-4-hydroxyanisole (BHA);    Rhodamine 123;    Mitochondrial respiratory chain;    Trypanosoma cruzi;   

DOI  :  10.1016/0014-5793(92)80480-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Results obtained with in situ mitochondria or Trypanosoma cruzi showed that this protozoon had only two energy coupling sites, sites II and III that correspond to higher eukaryote mitochondria. Rotenone did not inhibit the oxygen uptake or the parasite. These results suggest that the NADH-ubiquinone segment of the respiratory chain has no activity. Studies with in situ mitochondria confirmed that BHA, an antioxidant food additive, blocks the mitochondrial electron transport chain at the succinate-cytochrome b segment being the molecular basis of this trypanocidal action.

【 授权许可】

Unknown