FEBS Letters | |
The mitochondrial probe rhodamine 123 inhibits in isolated hepatocytes the degradation of short‐lived proteins | |
Knecht, E.1  Roche, E.1  Aniento, F.1  Vargas, J.L.1  Grisoli´a, S.1  | |
[1] Instituto de Investigaciones Citolo´gicas de la Caja de Ahorros de Valencia, Centro asociado al Consejo Superior de Investigaciones Cienti´ficas, Amadeo de Saboya, 4, 46010 Valencia, Spain | |
关键词: Rhodamine 123; Isolated hepatocyte; Short-lived protein; Protein degradation; | |
DOI : 10.1016/0014-5793(88)80438-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The fluorescent dye rhodamine 123 (R123) decreases the intracellular ATP levels and also inhibits the degradation of short-lived proteins in isolated hepatocytes. This inhibition affects lysosomal and, to some extent, non-lysosomal mechanisms. The degradation of short-lived proteins decreases more when ATP levels are less than 40% of those in control cells, in contrast to the reported linear correlation between ATP levels and degradation of long-lived proteins. R123 provides a powerful probe for clarifying the proteolytic mechanisms involved in degradation of short-lived proteins and the ATP requirements in protein degradation. Indeed, as illustrated, the results suggest different mechanisms for the degradation of short- and long-lived proteins. Moreover, they provide a warning for the clinical use of this reagent.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020290682ZK.pdf | 327KB | download |