The mitochondrial probe rhodamine 123 inhibits in isolated hepatocytes the degradation of short‐lived proteins

FEBS Letters

Knecht, E.¹  Roche, E.¹  Aniento, F.¹  Vargas, J.L.¹  Grisoli´a, S.¹ 

[1] Instituto de Investigaciones Citolo´gicas de la Caja de Ahorros de Valencia, Centro asociado al Consejo Superior de Investigaciones Cienti´ficas, Amadeo de Saboya, 4, 46010 Valencia, Spain

关键词: Rhodamine 123;    Isolated hepatocyte;    Short-lived protein;    Protein degradation;   

DOI  :  10.1016/0014-5793(88)80438-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The fluorescent dye rhodamine 123 (R123) decreases the intracellular ATP levels and also inhibits the degradation of short-lived proteins in isolated hepatocytes. This inhibition affects lysosomal and, to some extent, non-lysosomal mechanisms. The degradation of short-lived proteins decreases more when ATP levels are less than 40% of those in control cells, in contrast to the reported linear correlation between ATP levels and degradation of long-lived proteins. R123 provides a powerful probe for clarifying the proteolytic mechanisms involved in degradation of short-lived proteins and the ATP requirements in protein degradation. Indeed, as illustrated, the results suggest different mechanisms for the degradation of short- and long-lived proteins. Moreover, they provide a warning for the clinical use of this reagent.

【 授权许可】

Unknown