Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase

FEBS Letters

Dixon, Jack E.²  Powell, Steven M.¹  Zalkin, Howard¹ 

[1] Department of Biochemistry, Purdue University, West Lafayette, IN 49707, USA;Department of Biological Chemistry, The University of Michigan, Ann Arbor, MI 48109, USA

关键词: Recombinant DNA;    De novo purine nucleotide synthesis;    Gout;    Ade-H;    adenylosuccinate lyase gene;    AMP;    adenosine monophosphate;    AS;    adenylosuccinate synthetase;    ASS;    arginosuccinate synthetase;    ATP;    adenosine triphosphate;    bp;    base pair;    cAMP;    cyclic adonosine monophosphate;    cGMP;    cyclic guanosine monophosphate;    CHO;    Chinese Hamster Ovary;    GDP;    guanosine diphosphate;    GTP;    guanosine monophosphate;    IMP;    inosine monophosphate;    kb;    kilobase(s);    kDa;    kilodalton(s);    nt;    nucleotide;    PCR;    polymerase chain reaction;    Pi;    inorganic phosphate;    PPi;    pyrophosphate;    Pipes;    piperazine-N;    N′-bis[2-ethane-sulfonic acid]);    purA;    E. coli gene encoding adenylosuccinate synthetase;    SDS;    sodium dodecyl sulfate;    SSC;    sodium chloride;    sodium citrate;    SSPE;    sodium chloride;    sodium phosphate containing EDTA;   

DOI  :  10.1016/0014-5793(92)80465-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adenylosuccinate synthetase (AS) catalyzes the first committed step in the conversion of IMP to AMP. A cDNA was isolated from a human liver library which encodes a protein of 455 amino acids (M _r of 49.925). Alignments of human, mouse, Dictyostelium discoideum and E. coli AS sequences identify a number of invariant residues which are likely to be important for structure and/or catalysis. The human AS sequence was also 19% identical to the human urea cycle enzyme, arginosuccinate synthetase (ASS), which catalyses a chemically similar reaction. Both human liver and HcLa AS mRNA showed signals of 2.3 and 2.8 kb. An unmodified N-terminus is required for function of the human AS enzyme in E. coli mutants lacking the bacterial enzyme. The human cDNA provides a means to assess the possible role of AS abnormalities in unclassified, idiopathic cases of gout.

【 授权许可】

Unknown