FEBS Letters | |
Translation enhancing properties of the 5′‐leader of potato virus X genomic RNA | |
Miroshnichenko, N.A.2  Zelenina, D.A.2  Fedorkin, O.N.1  Atabekov, J.O.1  Morozov, S.Yu.1  Solovyev, A.G.1  Smirnyagina, E.V.1  Rodionova, N.P.1  Kulaeva, O.I.1  | |
[1] Department of Virology, Moscow State University, Moscow 119899, USSR;Institute of Microbiology, Academy of Sciences of the USSR, Moscow 117811, USSR | |
关键词: Recombinant DNA; Translational enhancement; Plant virus; NPT; neomycin phosphotransferase; PVX; potato virus X; GUS; β-glucuronidase; | |
DOI : 10.1016/0014-5793(92)80301-V | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The double-stranded DNA copy corresponding to the 5′-nontranslated αβ-leader of potato virus X (PVX) genomic RNA (positions −3 to −85 according to AUG initiator) was chemically synthesized and fused to the transcription plasmids containing three different reporter genes: neomycinphosphotransferase type II (NPT II) gene, Bacillus thuringiensis coleopteran-specific toxic protein gene and β-glucuronidase (GUS) gene. Expression of the reporter genes in vitro and in plant protoplasts (in the case of GUS gene) reveals that the αβ-leader of PVX RNA acts as a translation enhancer despite the presence of the upstream vector-derived sequence and irrespective of the length of the spacer sequence preceding the reporter genes.
【 授权许可】
Unknown
【 预 览 】
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