Translation enhancing properties of the 5′‐leader of potato virus X genomic RNA

FEBS Letters

Miroshnichenko, N.A.²  Zelenina, D.A.²  Fedorkin, O.N.¹  Atabekov, J.O.¹  Morozov, S.Yu.¹  Solovyev, A.G.¹  Smirnyagina, E.V.¹  Rodionova, N.P.¹  Kulaeva, O.I.¹ 

[1] Department of Virology, Moscow State University, Moscow 119899, USSR;Institute of Microbiology, Academy of Sciences of the USSR, Moscow 117811, USSR

关键词: Recombinant DNA;    Translational enhancement;    Plant virus;    NPT;    neomycin phosphotransferase;    PVX;    potato virus X;    GUS;    β-glucuronidase;   

DOI  :  10.1016/0014-5793(92)80301-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The double-stranded DNA copy corresponding to the 5′-nontranslated αβ-leader of potato virus X (PVX) genomic RNA (positions −3 to −85 according to AUG initiator) was chemically synthesized and fused to the transcription plasmids containing three different reporter genes: neomycinphosphotransferase type II (NPT II) gene, Bacillus thuringiensis coleopteran-specific toxic protein gene and β-glucuronidase (GUS) gene. Expression of the reporter genes in vitro and in plant protoplasts (in the case of GUS gene) reveals that the αβ-leader of PVX RNA acts as a translation enhancer despite the presence of the upstream vector-derived sequence and irrespective of the length of the spacer sequence preceding the reporter genes.

【 授权许可】

Unknown