Structural analysis of the putative regulatory region of the rat gene encoding poly(ADP‐ribose) polymerase

FEBS Letters

Dandenault, Brigitte¹  Kirkland, James B.¹  Duchaine, Caroline¹  Potvin, Frédéric¹  Thibodeau, Jacques¹  Poirier, Guy G.¹ 

[1] Laboratory du métabolisme du poly (ADP-ribose), Endocrinologie moléculaire, Centre Hospitalier de l'Université Laval, 2705 Boul, Laurier, Ste-Foy, Québec GIV 4G2, Canada

关键词: Poly(ADP-ribose) polymerase;    Genomic DNA cloning;    Nucleotide sequence;    Regulatory region;    Spl;    PARP;    poly(ADP-ribose) polymerase;    kDa;    kilo Dallons;    kbp;    kilo base pair(s);    cAMP;    cyclic AMP;   

DOI  :  10.1016/0014-5793(92)80457-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A γ EMBL3 clone containing the first three exons along with part of the 4th exon of the rat poly(ADP-ribose) polymerase gene was isolated from a genomic DNA library. This clone also contains 6.6 kbp of upstream sequences. Nucleotide sequence analysis of the proximal 5′ 670 nucleotides flanking the major RNA start site of the rat gene does not reveal significant global homology with the same region of the human gene, but a series of short sequences are identical. Among these sequences are found two putative Spl binding sites along with a decanucleotide sequence responsible for the attachment of the transcription factor AP-2.

【 授权许可】

Unknown