Nucleotide sequence of a cDNA clone encoding a major allergenic protein in rice seeds Homology of the deduced amino acid sequence with members of α‐amylase/trypsin inhibitor family

FEBS Letters

Izumi, Hidehiko¹  Tanaka, Kunisuke⁴  Fujii, Noboru¹  Kurosawa, Yoshikazu²  Urisu, Atsuo³  Matsuda, Tsukasa¹  Adachi, Takahiro¹  Nakamura, Ryo¹ 

[1] Department of Food Science and Technology, School of Agriculture, Nagoya University, Nagoya, Aichi 464-01, Japan;Institute for Comprehensive Medical Science, Fujita-Gakuen Health University, Toyoake, Aichi 470-11, Japan;Department of Pediatrics, Fujita-Gakuen Health University, Toyoake, Aichi 470-11, Japan;Department of Biochemistry, College of Agricultural Chemistry, Kyoto Perfectural University, Kyoto, Kyoto 606, Japan

关键词: Rice allergen;    Seed protein;    cDNA cloning;    α-Amylase-inhibitor family;    Sequence homology;   

DOI  :  10.1016/0014-5793(92)80443-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone of rice major allergenic protein (RAP) was isolated from a cDNA library of maturing rice seeds. The cDNA had an open reading frame (486 nucleotides) which coded a 162 amino acid residue polypeptide comprising a 27-residue signal peptide and a 135-residue mature protein of M_r 14,764. The deduced amino acid sequence of RAP showed a considerable similarity to barley trypsin inhibitor [1983, J. Biol. Chem. 258, 7998–8003] and wheat α-amylase inhibitor [1981, Phytochemistry 20, 1781–1784].

【 授权许可】

Unknown