Identification of γ‐type hordeins in barley

FEBS Letters

Kreis, M.²  Parmar, S.²  Shewry, P.R.²  Kasarda, D.D.¹  Lew, E.J.-L.¹ 

[1] Western Regional Research Centre, ARS United States Department of Agriculture, Berkeley, CA 94710, USA;Rothamsted Experimental Station, Harpenden, Herts, England

关键词: Barley;    Seed protein;    Amino acid sequence;    Homology;   

DOI  :  10.1016/0014-5793(85)80427-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The barley mutant Risϕ 56 has a deletion of at least 85 kb of DNA at the Hor 2 locus, resulting in the absence from the seeds of almost all of the major B hordein group of storage proteins. We have purified two fractions containing the three remaining ‘B hordein-like’ bands and determined their N-terminal amino acid sequences. One fraction, containing the two bands with higher M _r, gave a single major N-terminal sequence which was closely related to those reported for γ-type prolamins of wheat and rye. The sequence of the third band was also homologous with those of the γ-type prolamins, but less closely so. We consider that the γ-type prolamins are most closely related to the ancestral S-rich prolamins of the Triticeae, and this is the first report of their presence in barley.

【 授权许可】

Unknown