Effective detergent/lipid ratios in the solubilization of phosphatidylcholine vesicles by Triton X‐100

FEBS Letters

Goñi, Félix M.¹  Partearroyo, M.Aránzazu¹  Urbaneja, M.Angeles¹ 

[1] Department of Biochemistry, University of the Basque Country, PO Box 644, 48080 Bilbao, Spain

关键词: Surfactant;    Detergent;    Membrane solubilization;    Liposome;    Triton X-100;    SUV;    small unilamellar vesicles;    LUV;    large unilamellar vesicles;    MLV;    multilamellar vesicles;    R c;    effective ratio;    molar ratio of detergent/lipid in the mixed aggregates (vesicles or micelles);    R SAT c;    R c value at which the vesicles are saturated with the detergent;    R SOL c;    R c value at which the solubilization of the lipid is completed;    D w;    detergent concentration in the aqueous phase;   

DOI  :  10.1016/0014-5793(92)80424-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Effective detergent:lipid ratios (i.e. molar ratios in the mixed aggregates, vesicles or micelles) have been estimated for the solubilization of phosphatidylcholine vesicles by Triton X-100. Effective molar ratios are given for both the onset and the completion of bilayer solubilization; small unilamellar, large unilamellar and multilamellar vesicles have been used. Effective detergent:lipid ratios are independent of phospholipid concentration, and their use allows a deeper understanding of membrane-surfactant interactions.

【 授权许可】

Unknown