Expression of c‐met proto‐oncogene in COS cells induces the signal transducing high‐affinity receptor for hepatocyte growth factor

FEBS Letters

Higuchi, Osamu²  Vande Woude, George F.¹  Mizuno, Kensaku²  Nakamura, Toshikazu² 

[1] ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD 21701, USA;Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812, Japan

关键词: Hepatocyte growth factor;    HGF;    HGF receptor;    c-met Proto-oncogene;    Mitogenic signal;   

DOI  :  10.1016/0014-5793(92)80257-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By transfection of the expression plasmid containing a human c-met cDNA into COS-7 cells, high-affinity binding sites specific for HGF with a K _d value of 30 pM were newly detected. Furthermore, only in the c-met transfected COS-7 cells, but not in the control COS-7 cells, DNA synthesis was markedly induced in response to HGF. Thus, transient expression of exogenous c-met cDNA resulted in the appearance of high-affinity receptor for HGF and conversion of the normally non-responsive COS-7 cells into the HGF-responsive cells. These results provide evidence for identifying the c-met product as a signal transducing high-affinity receptor for HGF.

【 授权许可】

Unknown