Transforming growth factors type‐β and dexamethasone attenuate group II phospholipase A2 gene expression by interleukin‐1 and forskolin in rat mesangial cells

FEBS Letters

Pignat, Werner³  Geiger, Thomas¹  Märki, Fritz¹  McMaster, Garry¹  Pfeilschifter, Josef³  van den Bosch, Henk²  Mühl, Heiko³  Cerletti, Nico¹  Cox, David¹  Vosbeck, Klaus¹ 

[1] Research Department, Pharmaceuticals Division, Ciba-Geigy Ltd., CH-4002 Basel, Switzerland;Centre for Biomembranes and Lipid Enzymology, 3584 CH Utrecht, The Netherlands;Department of Pharmacology, Biocenter, University of Basel, CH-4056 Basel, Switzerland

关键词: Phospholipase A2;    Interlaukin-1;    Transforming growth factor β;    Dexamethasone;    Phospholipase A2 mRNA;    Mesangial cell;    PLA2;    phospholipase A2;    IL- 1;    interleukin-1;    TNF;    tumour necrosis factor;    TGF;    transforming growth factor;   

DOI  :  10.1016/0014-5793(92)81245-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of rat mesangial cells with interleukin-1β(IL-1β) and forskolin induced, in a synergistic fashion,the expression of group II phospholipase A₂ (PLA₂) mRNA, with subsequent increased synthesis and secretion of PLA₂. In contrast, interleukin-6 did not increase PLA₂ mRNA levels of PLA₂ activity. Transforming growth factor (TGF) β₁, TGFβ₂ and TGFβ₃ equipotently attenuated the IL-1β- and forskolin-induced elevation of PLA₂ mRNA, as well as PLA₂ synthesis and secretion. The glucocorticoid dexamethasone only partially suppressed the IL-1β- and forskolin-induced elevation of PLA₂ mRNA, but totally inhibited PLA₂ synthesis and secretion.

【 授权许可】

Unknown