Expression cloning of a sheep adreno‐ferredoxin using the polymerase chain reaction

FEBS Letters

Ichikawa, Yoshiyuki¹  Tsujita, Maki¹  Matsuo, Yoshinori¹  Mizoguchi, Kazuhiro¹ 

[1] Department of Biochemistry, Kagawa Medical School, Miki-cho, Kita-gun, Kagawa 761-07 Japan

关键词: Forredoxin;    Iron—sulfur protein;    Norheme iron protein;    Miltchondrial cytochrome P-430-linked monooxygenase;    PCR;    Expression cloning;   

DOI  :  10.1016/0014-5793(92)81232-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In addition to the selective amplification of cDNA from total RNA by the PCR method, the distinctive properties of ferredoxin-expressing colonies can be used for cloning a ferredoxin cDNA. This strategy for cloning and expressing cDNA in E. coli was applied to a sheep adreno-ferredoxin. The expressed sheep ferredoxin showed a spectral pattern typical or [2Fe-2S] proteins. The amino acid sequence deduced from the DNA sequence showed that the mature form of sheep ferredoxin consists of 128 amino acid residues. This rapid and simple method for cloning and expressing cDNA can be applied to other ferredoxins.

【 授权许可】

Unknown