Resonance Raman spectroscopic study on the iron—sulfur proteins containing [2Fe—2S] clusters

FEBS Letters

Hase, Toshiharu²  Nagayama, Kuniaki³  Ozaki, Yukihiro¹  Matsubara, Hiroshi²  Kyogoku, Yoshimasa¹ 

[1] Institute for Protein Research, Osaka University, Suita, Osaka 565, Japan;Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan;Department of Physics, Faculty of Science, University of Tokyo, Bunkyo-ku Tokyo 113, Japan

关键词: Resonance Raman spectroscopy;    Iron—sulfur protein;    Ferredoxin;    [2Fe-2S] cluster;   

DOI  :  10.1016/0014-5793(83)80387-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Resonance Raman spectra have been obtained for Spinacea oleracea, Phytolacca americana and Halobacterium halobium ferrodoxins. These spectra were very similar to each other in terms of both vibrational frequencies and relative intensities, strongly suggesting that their molecular structures of active centers are very similar. The resonance Raman spectra were, however, fairly different in the frequencies of Fe—S(Cys) stretching modes from the spectrum of beef adrenodoxin previously reported. Probably the nature of Fe—S(Cys)-bonding and/or the manner of cysteine ligation is somewhat different between adrenodoxin and the other [2Fe—2S] ferrodoxins.

【 授权许可】

Unknown