Identification and characterization of urokinase receptors in natural killer cells and T‐cell‐derived lymphokine activated killer cells

FEBS Letters

Andreasen, Peter A.¹  Petersen, Claus Munck²  Nykjær, Anders³  Gliemann, Jørgen³  Møller, Bjarne² 

[1] Institute of Molecular Biology, University of Aarhus, DK-8000 Aarhus C, Denmark;Department of Clinical Immunology, Skejby University Hospital, DK-8200, Aarhus N, Denmark;Institute of Medical Biochemistry, University of Aarhus, DK-8000 Aarhus C, Denmark

关键词: Urokinase receptor;    Lymphocyte;    LAK cell;    Internalization;    Plasminogen activator inhibitor;    Activation antigen;    DFP;    diisopropylflourophosphate;    [125I]DFP-uPA;    125I-labeled and DFP-treated uPA;    FACS;    flourescence-activated cell scanning;    LAK cells;    lymphokine activated killer-cells;    LGL;    large granular lymphocytes;    PAI-I;    plasminogen activator inhibitor type-i;    PPD;    purified protein derivative;    uPA;    urokinase type plasminogen activator;    uPAR;    receptor for uPA;   

DOI  :  10.1016/0014-5793(92)80154-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flourescence-activated cell scanning analysis of human blood cells revealed novel urokinase receptors in large granular lymphocytes and a small subset of T-cells (CD3+). Culturing of T-cells with interleukin-2 to generate CD3+ lymphokine-activated killer cells caused a large increase in urokinase binding, suggesting that the urokinase receptor is an activation antigen. The receptor in lymphocytes was similar to that in monocytes with regard to size, affinity and ligand specificity, but did not mediate degration of urokinase-inhibitor complexes. It is suggested that lymphocyte-bound pro-urokinase is activated, e.g. by the human T-cell specific serine proteinase. HuTSP-1, and thereby starts a cascade of plasminogen activation important for extravasation of the cells.

【 授权许可】

Unknown