Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A‐acyl carrier protein transacylase of Escherichia coli

FEBS Letters

Magnuson, Kelly¹  Larson, Timothy J.²  Cronan, John E.¹  Oh, Won² 

[1] Departments of Microbiology, University of Illinois, Urbana, IL 61801, USA;Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0308, USA

关键词: Malonyl transacylase;    Malonyl-CoA;    Acyl transferase;    Fatty acid biosynthesis;   

DOI  :  10.1016/0014-5793(92)80128-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli, Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain, Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids.

【 授权许可】

Unknown