Human centrosomal epitope is shared specifically with human lactate dehydrogenase‐B isozyme

FEBS Letters

Li, S.S.L.¹  Gosti, F.³  Bornens, M.³  Maunoury, R.² 

[1] Laboratory of Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA;Laboratoire d'Anatomie Pathologique, Centre Hospitalier Sainte-Anne, 75674 Paris, France;Centre de Génétique Moléculaire, CNRS, 2 Av. de la Terrasse, 91190, Gif-sur-Yvette, France

关键词: LDH-B;    Centrosome;    Human hepatic cell;   

DOI  :  10.1016/0014-5793(92)80121-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A rabbit serum (0013) used to identify pericentriolar proteins from isolated centrosomes (Gosti-Testu, F., Marty, M.C., Berges, J., Maunoury, R. and Bornens, M. (1986) EMBO J. 5, 2545–2550) was shown also to react through the same epitope with several non-centrosomal proteins including a major 36 kDa cytosolic antigen. This protein was identified to be human lactate dehydrogenase and the co-distribution of 0013 epitope on the centrosomal protein and on lactate dehydrogenase (LDH) was shown to be specific for human cells (Gosti, F., Marty, M.C., Courvalin, J.C., Maunoury, R. and Bornens, M. (1987) Proc. Natl. Acad. Sci. USA 84, 1000–1004). Human hepatic cells constitute, so far, the only exception to this co-distribution rule. By using this cell type which expresses only the LDH-A4 isozyme, we demonstrate that 0013 epitope is specific for the human LDH-B subunit, making serum 0013 the strongest anti-LDH-B available so far. The evolutionary and physiological significance of this situation is discussed.

【 授权许可】

Unknown