Induction of zinc metallothionein by calcium ionophore in vivo and in vitro

FEBS Letters

Garrett, Scott H.¹  Brady, Frank O.¹  Xiong, Xiaoyan¹  Arizono, Koji¹ 

[1] Department of Biochemistry and Molecular Biology, University of South Dakota School of Medicine, Vermillion, SD 57069, USA

关键词: Metallothionein;    Metallothionein mRNA;    A23187;    Calcium ionophore;   

DOI  :  10.1016/0014-5793(92)80245-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The calcium ionophore, A23187, can induce rat hepatic metallothionein (MT) when administered in vivo (5.8-fold, 5.0 μM, 11 h) and rat hepatocyte MT when administered in vitro (10.70-fold, 1.0 μM, 24 h). Several rat hepatoma cell lines (2M, 4.55-fold; JM2, 12.29-fold; EC3, 14.12-fold; HTC, 7.99-fold) and a normal rat liver cell line (Clone 9, 39.67-fold) were tested for their inducibility of MT mRNA by Cd²⁺ (10 μM, 8 h). Quantitatively, JM2 and 2M made the most MT mRNA, while HTC made the least. A23187 (0.1–7.0 μM) was studied as an inducer of MT mRNA in these cell lines (except for HTC) and in HeLa. A variety of responses and tolerances were seen with inductions ranging up to 32.11-fold. Quantitatively, the best responding cell lines were EC3 and 2M. A combination induction experiment, using TPA, a protein kinase C activator, and A23187 in EC3 cells revealed an additive effect of the two inducers on MT mRNA levels: TPA (10 nM), 11.71-fold; A23187 (3.0μM), 6.71-fold; and TPA + A23187, 20.00-fold. These studies have implicated perturbations in cytosolic calcium ion concentrations, caused by the ionophore A23187, as being involved in the complicated signaling systems which can lead to induction of MT mRNA and protein.

【 授权许可】

Unknown