Effects of the calcium ionophore A 23187 on low‐density lipoprotein processing and lipid metabolism in cultured human fibroblasts

FEBS Letters

Mazière, J.C.¹  Mora, L.¹  Polonovski, J.¹  Mazière, C.¹  Gardette, J.¹ 

[1] Laboratoire de Biochimie et ERA 481 du CNRS, CHU Saint-Antoine, 27 rue Chaligny, 75012 Paris, France

关键词: Calcium ionophore;    A 23187;    LDL internalization;    Lipid synthesis;    (Human fibroblast);   

DOI  :  10.1016/0014-5793(84)80984-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pretreatment of cultured human fibroblasts with the calcium ionophore A 23187 resulted in a decrease in low-density lipoprotein internalization. This effect was dose-dependent and did not occur in a medium devoid of calcium. About 2-fold reduction was observed with 10⁻⁵ M A 23187. In contrast, the low-density lipoprotein binding was only slightly affected. The incorporation of [¹⁴C]acetate and [¹⁴C]oleate into all classes of lipids (sterol, triacylglycerols and phospholipids) was strikingly reduced by ionophore pretreatment.

【 授权许可】

Unknown