Genomic organization and analysis of the 5′ end of the porcine ryanodine receptor gene (ryr1)

FEBS Letters

Brenig, Bertram¹  Brem, Gottfried¹ 

[1] Lehrstuhl für Molekulare Tierzucht, Veterinärmedizinische Fakultät der Ludwig-Maximilians-Universität München, Veterinärstrasse 13, D-8000 Munich 33, Germany

关键词: Ryanodine receptor;    Malignant hyperthermia;    Stress syndrome;    Halothane;    Volatile anaesthetics;    Sus scrofa domestica;   

DOI  :  10.1016/0014-5793(92)80076-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study we describe the isolation of genomic clones of the 5′ region of the porcine ryanodine receptor gene, a candidate for malignant hyperthermia in pigs and humans. The recombinants were isolated from a porcine liver, genomic DNA library in phage EMBL3A after screening with PCR amplified DNA fragments. The exon/intron structure of the ryanodine receptor gene was determined by DNA sequencing. Based on the sequence data it was possible to develop a simple test for the detection of malignant hyperthermia susceptible and normal pigs.

【 授权许可】

Unknown