Human melanotransferrin (p97) has only one functional iron‐binding site

FEBS Letters

Hellström, Karl Erik¹  Stebbins, Mark R.¹  Hellström, Ingegerd¹  Baker, Edward N.²  Baker, Heather M.²  Kahn, Maria¹  Smith, Clyde A.² 

[1] Bristol-Myers Squibb, 3005 First Avenue, Seattle, WA 98121, USA;Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand

关键词: Iron binding;    Melanotransferrin;    Transferrin;    Melanoma cell antigen;   

DOI  :  10.1016/0014-5793(92)80060-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The iron-binding properties of melanotransferrin, the tumour-associated antigen also known as p97, have been investigated by UV/visible and fluorescence spectroscopy, amino acid sequence comparison, and modelling. These show that, in contrast to other transferrins, melanotransferrin binds only one Fe³⁺ ion per molecule. The binding properties of its N-terminal site are similar to other transferrins, but its C-terminal site does not bind iron at all. The differences can be related to specific amino acid changes in the C-terminal site.

【 授权许可】

Unknown