Interaction of calmodulin with lactoferrin

FEBS Letters

Tejerina, J.M.²  de Lillo, A.¹  Fierro, J.F.¹ 

[1] Departamento de Biologia Funcional, Facultad de Medicina, Escuela de Estomatologia, Universidad de Oviedo, 33006 Oviedo, Spain;Departamento de Biologia Funcional, Servicio de Periodoncia, Escuela de Estomatologia, Universidad de Oviedo, 33006 Oviedo, Spain

关键词: Calmodulin;    Lactoferrin;    Ca2+-binding protein;    Lactotransferrin;   

DOI  :  10.1016/0014-5793(92)80055-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calmodulin, as a major intracellular calcium-binding protein, regulates many Ca²⁺-dependent enzymes and plays an important role in a wide spectrum of cellular functions of the eukaryotes. Interaction between calmodulin and human lactoferrin, a 78 kDa protein with antibacterial properties, was found in the presence of Ca²⁺ using (i) a method for the detection of calmodulin binding proteins with biotinylated calmodulin, (ii) affinity chromatography on an agarose—calmodulin column with subsequent detection by an enzyme-linked immunosorbent assay (ELISA). The binding of calmodulin to lactoferrin blocked the ability of lactoferrin to agglutinate Micrococcus lysodeikticus.

【 授权许可】

Unknown