Molecular characterization of a calmodulin‐like Dictyostelium protein CalB

FEBS Letters

Smýkal, Petr²  Folk, Petr¹  Rösel, Daniel¹  Půta, František¹  Blahůšková, Anna¹ 

[1] Department of Physiology and Developmental Biology, Charles University, Viničná 7, Praha 2 128 00, Czech Republic;Department of Plant Physiology, Charles University, Viničná 7, Praha 2 128 00, Czech Republic

关键词: Ca2+-binding protein;    Calmodulin;    EF-hand;    Dictyostelium discoideum;    CaM;    calmodulin;    BsR;    blasticidin resistance;   

DOI  :  10.1016/S0014-5793(00)01551-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A gene named calB was cloned and characterized in Dictyostelium. A relationship to calmodulin (CaM) is suggested by sequence identity (50%), similar exon–intron structure and cross-reactivity with anti-CaM sera. The level of calB mRNA is developmentally regulated with maxima during aggregation and in spores. CalB null cells grow normally, develop and produce viable spores. We demonstrated the capacity of tagged CalB to bind Ca²⁺ using the ⁴⁵Ca²⁺ overlay assay and showed that its mobility on SDS–PAGE is dependent on Ca²⁺/EGTA pretreatment.

【 授权许可】

Unknown