Synthesis of α1‐microglobulin in cultured rat hepatocytes is stimulated by interleukin‐6, leukemia inhibitory factor, dexamethasone and retinoic acid

FEBS Letters

Pierzchalski, Piotr¹  Åkerström, Bo³  Koj, Aleksander¹  Fries, Erik²  Rokita, Hanna¹ 

[1] Institute of Molecular Biology, Jagiellonian University, 31-120 Krakow, Poland;Department of Medical and Physiological Chemistry, University of Uppsala, Biomedical Center, S-75123 Uppsala, Sweden;Department of Medical and Physiological Chemistry, University of Lund, S-22100 Lund, Sweden.

关键词: Interleukin-6;    Retionoic acid;    Rat hepatocyte;    Acute phase;    α1-Microglobulin;    Bikunin;    α1-m;    α1-microglobulin;    IL-6;    interleukin-6;    LIF;    leukemia inhibitory factor;    CPI;    cysteine proteinase inhibitor;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(92)80047-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The secretion or α₁-microglobulin by primary cultures of rat hepatocytes was found to increase upon the addition of interleukin-6 or leukemia inhibitory factor, two mediators of acute phase response. This stimulatory effect was further enhanced by dexamethasone. α₁-Microglobulin is synthesized as a precursor also containing bikunin, and the precursor protein is cleaved shortly berore secretion. Our results therefore suggest that both α₁-microglobulin and bikunin are acute phase reactants in rat hepatocytes. Furthermore, we found that retinoic acid, previously shown to be involved in the regulation of cell differentiation and development, also stimulated α₁-microglobulin synthesis. Only free, uncomplexed α₁-microglobulin (28,000 Da) was detected in the hopatocyte media, suggesting that the complex between α₁-microglobulin and α₁-inhibitor 3, found in rat serum, is formed outside the hepatocyte.

【 授权许可】

Unknown