Molecular cloning and nucleotide sequence determination of the regulator region of mecA gene in methicillin‐resistant Staphylococcus aureus (MRSA)

FEBS Letters

Asada, Kazumi²  Okonogi, Kenji¹  Suzuki, Eiko²  Yokota, Takeshi²  Hiramatsu, Keiichi² 

[1] Central Research Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka, Japan;Department of Bacteriology, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan

关键词: mecA;    Repressor;    Co-inducer;    Methicillin resistance;    Staphylococcus aureus;   

DOI  :  10.1016/0014-5793(92)80039-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Molecular cloning and nucleotide sequence analysis were performed for the identification or the regulator genes of methicillin resistance in the genome of a MRSA strain N315. Two open reading frames (orfs) were identified in the 5′-flanking region of the mecA gene. Predicted amino acid sequences of these orfs showed extensive homology to the co-inducer and the repressor protein of the penicillinase (PCase) production in Staphylococcus aureus as well as in Bacillus licheniformis. These orfs are considered to encode putative co-inducer and repressor proteins specific for the regulation of methicillin resistance in MRSA.

【 授权许可】

Unknown