Thermoluminescence evidence for light‐induced oxidation of tyrosine and histidine residues in manganese‐depleted photosystem II particles

FEBS Letters

Klimov, Vyacheslav V.¹  Allakhverdiev, Suleyman I.¹  Demeter, Sandor² 

[1] Institute of Soil Science and Photosynthesis, USSR Academy of Sciences, Pushchino, Moscow Region, 142292, USSR;Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, Szeged, H-6701. Hungary

关键词: Histidine;    Tyrosine;    Photosystem II;    Electron transport;    Manganese-depletion;    Thermoluminescence;    DCPIP;    2;    2-dichlorophenol-indophenol;    DEPC;    die-thylpyrocarbonate;    DPC;    diphenylcarbazide;    DT-20;    oxygen evolving photosystem II particle;    FeCy;    ferricyanide;    His+;    redox active histidine residue;    NBD;    7-chloro-4-nitrobenz-2-oxa-1;    3-diazole;    QA;    primary quinone acceptor of photosystem II;    SiMo;    silicomolybdate;    PS II;    photosystem II;    Yz +;    tyrosine;    donor of PS II;   

DOI  :  10.1016/0014-5793(92)80325-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the thermoluminescence (TL) glow curve of photosystem II, particles depleted of manganese, a tyrosine modifier, 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD) a bolishes the TL band appearing around −55°C (TL₋₅₅). Addition of a histidine modifier, diethylpyrocarbonate results in the disappearance of the band peaking around −30°C (TL₋₃₀). NBD treatment also abolishes the EPR signal II_fast of oxidized tyrosine donor, Y₂₁ and inhibits the electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. It is concluded that the TL₋₅₅ and TL₋₃₀ bands can be assigned to oxidized tyrosine (Y₂ ⁺) and histidine (His⁺) residues, respectively, which participate in electron transfer from manganese to the reaction center of chlorophyll, P680⁺.

【 授权许可】

Unknown