PKC‐independent inhibition of glutamate exocytosis by arachidonic acid in rat cerebrocortical synaptosomes

FEBS Letters

Sánchez-Prieto, José¹  Miras-Portugal, María Teresa¹  Herrero, Inmaculada¹ 

[1] Departamento de Bioquimica, Facultad de Veterinaria, Universidad Complutense, Madrid 28040, Spain

关键词: Protein kinase C;    Glutamate exocytosis;    Arachidonic acid;    Synaptosome;    Rat brain;    4AP;    4-aminopyridine;    4β-PDBu;    4β-phorbol dibutyrate;    [Ca2+];    cytosolic free Ca2+ concentration;    TES;    2-([2-hydroxy-1;    1-bis(hydroxymethyl)-ethyl]-amino)ethane sulphonate;    TPA;    12-O-tetradecanoylphorbol 13-acetate;   

DOI  :  10.1016/0014-5793(92)80312-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In rat cerebrocortical synaptosomes, the addition of 4β-phorbol dibutyrate (4β-PDBu) and arachidonic acid enhances and decreases, respectively, the glutamate release evoked by 4-aminopyridine. Pretreatment of synaptosomes with 12-O-tetradecanoylphorbol 13-acetate (TPA) or preincubation with staurosporine, prevent the stimulatory effect of 4β-PDBu, but are without effect on the inibitory action of arachidonic acid. Moreover, methyl arachidonate, which is not effective as a PKC activator, also strongly inhibits glutamate exocytosis. These results suggest that PKC is not involved in the inhibition of glutamate release by arachidonic acid.

【 授权许可】

Unknown