Protein kinase C in rat brain synaptosomes

FEBS Letters

Tanaka, Shin-ichiro¹  Yasuda, Ichiro¹  Kishimoto, Akira¹  Tominaga, Masahiro¹  Nishizuka, Yasutomi¹ 

[1] Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan

关键词: Protein kinase C;    Synaptosome;    Membrane-skeleton;    Growth-associated protein-43;    Rat brain;    PKC;    protein kinase C;    GAP-43;    growth-associated protein-43;   

DOI  :  10.1016/0014-5793(91)81445-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A small fraction (approximately 5%) of protein kinase C (PKC) in the adult rat brain synaptosomes is tightly associated with Triton X-100-insoluble components (most likely membrane-skeleton elements), and is solubilized only after denaturation with sodium dodecyl sulfate. The kinase domain of this PKC can be released as a soluble form after limited proteolysis with calpain, whereas the regulatory domain which binds phorbol ester remains insoluble. The PKC in this fraction was identified as the βII-subspecies or its related molecule. Presumably, this enzyme subspecies is responsible for the phosphorylation of a major PKC substrate protein, growth-associated protein-43, which is located in nerve endings as well as in growth cones in association with the membrane-skeleton elements.

【 授权许可】

Unknown