Imidazole‐SDS‐Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins

FEBS Letters

Castellanos, Lila¹  Ortiz, Maria Luisa²  Patron, Carlos Fernandez¹  Mendez, Enrique²  Calero, Miguel² 

[1] Center for Genetic Engineering and Biotechnology, PO Box 6162, La Habana-6, Cuba;Servicio de Endocrinologia, Hospital Ramon y Cajal, 28034-Madrid, Spain

关键词: Imidazole;    Reverse staining;    Microsequencing;    PAGE;   

DOI  :  10.1016/0014-5793(92)80309-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A reverse staining procedure is described for the detection of proteins in acrylamide and agarose gels with and without SDS. Protein detection occurs a few minutes after electrophoresis. The sensitivity on acrylamide gels is higher than that of Coomassie blue staining either on acrylamide gels or on electrotransferred membranes. Sequencing of protein bands only detected by reverse staining on the gel and not by Coomassie blue is demonstrated.

【 授权许可】

Unknown