Primary structure of ovine pituitary basic fibroblast growth factor

FEBS Letters

Moritz, Robert L.¹  Rubira, Michael R.¹  Burgess, Antony W.²  Simpson, Richard J.¹  Nice, Edouard C.²  Fabri, Louis J.²  Lloyd, Christopher J.² 

[1] Joint Protein Structure Laboratory, Ludwig Institute for Cancer Research (Melbourne) and The Walter and Eliza Hall Institute o Medical Research PO Royal Melbourne Hospital, Victoria 3050, Australia;Ludwig Institute for Cancer Research (Melbourne), PO Royal Melbourne Hospital, Victoria 3050, Australia

关键词: Fibroblast growth factor;    Microsequencing;    Primary structure;    Ion-pair chromatography;    Microbore HPLC;   

DOI  :  10.1016/0014-5793(87)80435-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequence of basic FGF (146 residues) from ovine pituitary glands has been established. This has been achieved by the sequence analysis of subnanomole amounts of the intact molecule and of peptides derived by enzymatic digestions with clostripain, chymotrypsin, pepsin and Staphylococcus aureus V8 protease. Microbore HPLC, employing 1–2 mm i.d. columns, was used to purify, concentrate and buffer-exchange the FGF peptides. A novel application of ion-pairing chromatography was employed to isolate peptides which were not retained on conventional reversed-phase systems. There is only one positional difference between the ovine and bovine basic FGF_s, but there are 3 positional differences between ovine and human basic FGFS.

【 授权许可】

Unknown