FEBS Letters | |
Intracellular Ca2+ release by flufenamic acid and other blockers of the non‐selective cation channel | |
Poronnik, P.1  Cook, D.I.1  Ward, M.C.1  | |
[1] Department of Physiology, University of Sydney, NSW 2006, Australia | |
关键词: Non-selective cation channel; Intracellular calcium; Flufenamic acid; Patch clamp; Fura-2; | |
DOI : 10.1016/0014-5793(92)80296-S | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We report in this paper using measurement of intracellular free Ca2+ with fura-2, that flufenamic acid and several related blockers of the 25 pS Ca2+-activated nonselective cation channel cause release of Ca2+ from an intracellular store other than the endoplasmic reticulum, Possibly from mitochondria. A new compound, 4′-methyl-DPC, is found to be as effective in blocking non-selective cation channels as other flufenamate analogs but, like the parent compound, the non-selective cation channel blocker DPC, it does not cause release of Ca2+ from intracellular stores. DPC and 4′-methyl-DPC are thus the most suitable of the available blockers of non-selective cation channels for use in studies on the role of these channels in normal cell function.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020295846ZK.pdf | 384KB | download |