Intracellular Ca2+ release by flufenamic acid and other blockers of the non‐selective cation channel

FEBS Letters

Poronnik, P.¹  Cook, D.I.¹  Ward, M.C.¹ 

[1] Department of Physiology, University of Sydney, NSW 2006, Australia

关键词: Non-selective cation channel;    Intracellular calcium;    Flufenamic acid;    Patch clamp;    Fura-2;   

DOI  :  10.1016/0014-5793(92)80296-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report in this paper using measurement of intracellular free Ca²⁺ with fura-2, that flufenamic acid and several related blockers of the 25 pS Ca²⁺-activated nonselective cation channel cause release of Ca²⁺ from an intracellular store other than the endoplasmic reticulum, Possibly from mitochondria. A new compound, 4′-methyl-DPC, is found to be as effective in blocking non-selective cation channels as other flufenamate analogs but, like the parent compound, the non-selective cation channel blocker DPC, it does not cause release of Ca²⁺ from intracellular stores. DPC and 4′-methyl-DPC are thus the most suitable of the available blockers of non-selective cation channels for use in studies on the role of these channels in normal cell function.

【 授权许可】

Unknown