Anhydrosubtilisin‐catalyzed peptide synthesis

FEBS Letters

Sugimura, Akihiro¹  Kanaoka, Yuichi¹  Tanizawa, Kazutaka¹ 

[1] Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

关键词: Protease catalysis;    Peptide synthesis;    Inactive protease;    Anhydrosubtilisin;    Fragment condensation;   

DOI  :  10.1016/0014-5793(92)80370-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Anhydrosubtilisin was found to be a good catalyst for the peptide synthesis though its hydrolytic activity was fully inhibited. Amino acid (peptide) p-chlorophenyl ester (acyl donor) was coupled with amino acid (peptide) amide in the presence of the modified enzyme. The method did not afford by-products resulting from the hydrolysis of the acyl donor. This is the most advantageous characteristic of the method, since the hydrolysis of the acyl donor is unavoidable for the methods using catalytically active proteases. Anhydrosubtilisin was further shown to be useful for the fragment condensation of peptides.

【 授权许可】

Unknown