Retroviral‐mediated transfer of a dystrophin minigene into mdx mouse myoblasts in vitro

FEBS Letters

Dunckley, M.G.³  Morris, G.E.²  Dickson, G.³  Love, D.R.¹  Walsh, F.S.³  Davies, K.E.¹ 

[1] Molecular Genetics Group, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, UK;Research Division, NE Wales Institute, Deeside, Clwyd, CH5 4BR, UK;Department of Pathology, UMDS, Guy's Hospital, London, SE1 9RT, UK

关键词: Dystrophin: Recombinant retrovirus;    Gene transfer;    mdx mouse;    Skeletal muscle;   

DOI  :  10.1016/0014-5793(92)80363-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have demonstrated expression of a 6.3 kb Becker muscular dystrophy (BMD) human dystrophin cDNA following retroviral-mediated transduction of cultured myoblasts from the dystrophin-deficient mdx mouse. The truncated dystrophin protein was localised to the sarcolemma of differentiated myotubes by antibodies against the C-terminus of the molecule, and produced an identical immunostaining pattern to that observed in control myotubes expressing normal endogenous dystrophin. These results indicate that retroviral-mediated gene transfer may be useful for experimental in vivo studies on the complementation of dystrophin gene mutations.

【 授权许可】

Unknown