2‐Aminopurine inhibits RNA and protein synthesis and reduces catecholamine desensitization in C6‐2B rat glioma cells

FEBS Letters

DeBernardi, Maria A.¹  Brooker, Gary¹ 

[1] Department of Biochemistry and Molecular Biology, and Fidia-Georgetown Institute for the Neurosciences, Georgetown University School of Medicine, Washington, DC 20007, USA

关键词: c-fos;    Cell biosynthesis;    Refractoriness;    2-AP;    2-aminopurine;    Bt2cAMP;    N 0;    O2′-dibutyryladenosine 3′;    5′-cyclic monophosphate;    IBMX;    3-isobutyl-l-methylxanthine;   

DOI  :  10.1016/0014-5793(92)80415-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously proposed that intracellular cyclic AMP accumulation induces a putative, rapidly turning over protein inhibitory to further hormone activation of adenylate cyclase. In the present study, 2-aminopurine, which has been reported to selectively block c-fos gene expression, was used to test the hypothesis that c-fos protein might be involved in the desensitization process. Indeed, a reduction in heterologous desensitization to catecholamines was observed in 2-aminopurine-treated C6-2B rat glioma cells. However, we found 2-aminopurine to inhibit, in a concentration-dependent manner, total cellular RNA and protein synthesis in C6-2B. HeLa, Swiss 3T3 and BALB/e cells. mRNA synthesis was also markedly reduced in 2-aminopurine-treated cells. These unexpected findings, while supporting our hypothesis of a protein synthesis-sensitive step in the development of refractoriness, raise concern about the specificity of action of 2-aminopurine to inhibit c-fos induction and thus any cellular process, including desensitization, which might be regulated by c-fos gene expression.

【 授权许可】

Unknown