The presence of free D‐serine in rat brain

FEBS Letters

Takahashi, Kiyohisa³  Nishikawa, Toru³  Fujii, Noriko¹  Hashimoto, Atsushi³  Oka, Takae³  Harada, Kaoru¹  Hayashi, Tohiski² 

[1] Department of Chemistry, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan;Division of Biochemical and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-I Ogawa-Higashi, Kodaira, Tokyo, Japan;Division of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-I Ogawa-Higashi, Kodaira, Tokyo, Japan

关键词: D-Serine;    Amino acid enantiomer;    Chirasil-L-Val;    Gas chromatography;    Mass spectrometry;    Rat brain;    GC;    gas chromatography;    MS;    mass spectrometry;    PFPA;    pentafluoropropionic anhydride;    TCA;    trichloroacelic acid;    EI;    electron-impact ionization;    PCI;    positive chemical ionization;    NMDA;    N-methyl-D-aspartate;    L-NL;    L-norleucine;   

DOI  :  10.1016/0014-5793(92)80397-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Free amino acid enantiomers in adult rat brain extracts were analysed as their N,O-pentafluoropropionyl isopropyl derivatives by gas chromatography on a capillary column of Chirasil-L-Val. A peak X, which exhibited the same retention time as the N,O-pentafluoropropionyl isopropyl derivative of authentic D-serine, was detected in the brain extracts. Electron impact and positive chemical ionization mass spectra of the peak X of the brain extracts were identical to those of authentic D-serine. The concentration of free D-serine and the ratio of D-serine/total serine in the brain were estimated to be 0.27 and 0.23, υmol/g of wet weight, respectively. These data provide the first evidence that substantial quantities of free D-serine are present in mammalian brain tissues.

【 授权许可】

Unknown