Immunologic and structural relatedness of the integrin β7complex and the human intraepithelial lymphocyte antigen HML‐1

FEBS Letters

Mead, Paul¹  Watson, James D.¹  Ameratunga, Rohan V.¹  Krissansen, Geoffrey W.¹  Prestidge, Ross L.¹  Jenkins, David R.¹  Hollander, Daniel¹  Leung, Euphemia¹  Yuan, Qian¹  Print, Cristin G.¹  Yong, Rita¹  Cerf-Bensussan, Nadine²  Jiang, Wei-meng¹ 

[1] Department of Molecular Medicine, School of Medicine, University of Auckland, New Zealand;Group of Paediatric Immunology and Rheumatology, INSERM U132, Hopital des Enfant Malades, Paris, France

关键词: Integrin;    β7 subunit;    HML-1;    Protein structure;    BSA;    bovine serum albumin;    IEL;    interpithelial lymphocytes;    LPAM-1;    lymphocyte Peyer's patch adhesion molecule-1;    PBL;    peripheral blood lymphocyte;    PHA;    phytohaemagglutinin;    VLA;    very late antigen;    VnR;    vitronectin receptor;   

DOI  :  10.1016/0014-5793(92)80395-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently cloned the newest human integrin β subunit, termed β₇, from a cDNA library constructed from SEA-activated T lymphocytes. In this communication, we report on the structure of the human integrin β₇ protein complex determined using a rabbit anti-β₇ peptide antibody raised to an N-terminal 22 amino acid residue sequence deduced from the human β₇ subunit cDNA. The β₇ subunit (M, 116 000) expressed on PHA lymphoblasts associates with asingle major α subunit (α₁₁) that is distinct from the prominent T cell marker, integrin α₄. The α₁₁ subunit (M _r 180 000 nonreduced) displays a distinctive shift in size on reduction to an apparent M _r of 150 000. We show that these structural properties of the integrin β₇ complex are shared with the cell surrace antigen HML-1 found highly expressed on T cells which populate the intestinal epithelium and are proposed to be involved in mucosal immunity. Sequential immunoprecipitation and Western blotting demonstrate identity or close homology between the α₁₁β₇ and HML-1 proteins.

【 授权许可】

Unknown