Cloning and functional characterization of a cocaine‐sensitive dopamine transporter

FEBS Letters

Giros, Bruno¹  El Mestikawy, Salah¹  Caron, Marc G.¹  Bertrand, Lucie¹ 

[1] Departments of Cell Biology and Medicine and The Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, NC 27710, USA

关键词: Dopamine transporter;    Transmembrane domain;    Substantia nigra pars compacta;    Ventral tegmental;   

DOI  :  10.1016/0014-5793(91)81406-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the cloning of a rat cDNA encoding a functional dopamine transporter. This cDNA, derived from an intron-containing gene, encodes a protein of 620 amino acids. Hydropathicity analysis of the protein sequence suggests the presence of 12 putative transmembrane domains. The protein displays considerable identity with transporters for noradrenaline and GABA (64 and 30%, respectively). Transient expression of the cDNA in COS7 cells directs the expression of dopamine uptake activity with appropriate pharmacology and in a sodium-dependent fashion. In situ hybridization reveals that the mRNA for this transporter is expressed in the substantia nigra and ventral tegmental area, regions that contain dopaminergic cell bodies.

【 授权许可】

Unknown