Gene expression of type I phospholipase A2 in pancreatic beta cells

FEBS Letters

Metz, S.¹  Draznin, B.²  Robertson, R.P.³  Holmes, D.²  Leitner, W.² 

[1]Divisions of Endocrinology and Departments of Medicine of the University of Wisconsin and Middleton Memorial Veterans Hospital, Madison, WI, USA

[2]Denver VA Medical Center, Denver, CO, USA

[3]University of Minnesota, Minneapolis, MN, USA

关键词: Insulin;    Pancreatic islet;    Phospholipase;    Glucose;    Beta cell;    Fasting;    PLA2;    phospholipase A2;    cDNA;    complementary DNA;   

DOI  :  10.1016/0014-5793(91)81397-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A₂. The levels of phospholipase A₂ mRNA increased in islets from fasted rats: they decreased in islets cultured in a high glucose concentration (control values at 5.5 mM glucose = 150±6% of those at 22 mM) which impaired subsequent insulin secretion (reduction in second-phase release = 70±11%). These studies uniquely demonstrate that type I phospholipase A₂ is expressed specifically in beta cells and that nutrient availability modulates transcript levels, an effect which could contribute to the detrimental influence of prolonged hyperglycemia on islet function.

【 授权许可】

Unknown