Distinct effects of acetylcholine and glucose on 45calcium and 86rubidium efflux from mouse pancreatic islets

FEBS Letters

Henquin, J.C.¹  Awouters, P.¹  Nenquin, M.¹  Mathot, F.¹ 

[1] Unité de Diabétologie et Nutrition, University of Louvain Faculty of Medicine, UCL 54.74, B 1200 Brussels, Belgium

关键词: Pancreatic islet;    Acetylcholine;    Glucose;    Insulin release;    Calcium;    Ion efflux;   

DOI  :  10.1016/0014-5793(84)81218-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The similarities between the effects of acetylcholine and glucose on phospholipid metabolism in pancreatic islet cells prompted the comparison of their effects on ionic fluxes. Acetylcholine (1 μM) consistently increased ⁴⁵Ca²⁺ efflux from mouse islets, whereas glucose increased it in the presence, but decreased it in the absence of extracellular Ca²⁺. Acetylcholine consistently accelerated ⁸⁶Rb⁺ efflux, and this effect was augmented by Ca²⁺ omission. On the other hand, glucose markedly inhibited ⁸⁶Rb⁺ efflux, except when its concentration was raised from 10 to 15 mM in the presence of Ca²⁺. Unlike their effects on phospholipid metabolism, the ionic effects of the two insulin-secretagogues are thus very different.

【 授权许可】

Unknown