Dynamic light scattering studies of the aggregation of lysozyme under crystallization conditions

FEBS Letters

Skouri, Mohammed¹  Delsanti, Michel¹  Munch, Jean-Pierre¹  Giegé, Richard²  Lorber, Bernard² 

[1] Laboratoire d'Ultrasons et de Dynamique des Fluides Complexes, URA 851 du CNRS, Université Louis Pasteur, 4, rue Blaise Pascal, F-67070 Strasbourg Cedex France;Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS, 15, rue René Descartes, F-67084 Strasbourg Cedex, France

关键词: Dynamic light scattering;    Protein;    Lysozyme;    Supersaturation;    Aggregation;    Crystallization;    DLS;    dynamic light scattering;    ICF;    intensity autocorrelation function;   

DOI  :  10.1016/0014-5793(91)81391-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intensity autocorrelation functions of light scattered by lysozyme solutions under pre-crystallization conditions in NaCl-containing media were recorded at scattering angles from 20° to 90°. The measurements, conducted on freshly prepared protein solutions supersaturated more than 3-fold, indicate the simultaneous presence of two scatterer populations which can be assigned to individual protein molecules and to large particles. When solutions are undersaturated, or slightly supersaturated, light scattering only reveals the presence of the small scatterers. In the supersaturated medium, where aggregates were detected, lysozyme crystals grew in a time-span of 1–3 days after the scattering experiments. These results are correlated with the nucleation step during protein crystallization.

【 授权许可】

Unknown