Regulation by Ca2+ of membrane elasticity of bovine chromaffin granules

FEBS Letters

Fujime, Satoru¹  Miyamoto, Shigeaki¹ 

[1] Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194, Japan

关键词: Chromaffin granule;    Membrane elastic modulus;    Dynamic light scattering;    Osmotic swelling;    DLS;    dynamic light scattering;   

DOI  :  10.1016/0014-5793(88)80226-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In a range of [Ca²⁺] similar to cytosolic transient, a drastic reduction from about 20 dyn/cm to almost zero was observed in the membrane elastic modulus of bovine chromaffin granules, isolated in a solution containing 0.3 M sucrose and 5 mM Hepes at pH 7.0, and measured by combination of osmotic swelling and dynamic light-scattering (DLS) methods. This result suggests that the granule membrane becomes extremely flexible as a prelude to exocytosis.

【 授权许可】

Unknown