The major endogenous bovine brain protein kinase C inhibitor is a heat‐labile protein

FEBS Letters

Walsh, Michael P.¹  Fraser, Elaine D.¹ 

[1] MRC Group in Signal Transduction, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1

关键词: Protein kinase C inhibitor;    Bovine brain;    EGTA;    ethylenebis(oxyethylenenitrilo)tetraacetic acid;    PKC;    protein kinase C;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(91)81450-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A crude cytosolic fraction prepared from bovine brain contained protein kinase C, as shown by immunoblotting, but its activity was undetectable, suggesting the presence of interfering factors. Phosphatase, ATPase and protease activities did not account for the absence of detectable protein kinase C activity. The major contributing factor was found to be a heat-labile protein which was separated from the kinase by ion-exchange chromatography. The contribution to the total inhibitory activity of heat-stable proteins was relatively minor, suggesting that they may not function physiologically as protein kinase C inhibitors.

【 授权许可】

Unknown