Demonstration of L1‐related mRNAs in rat brain using DNA oligonucleotide probes

FEBS Letters

Gaardsvoll, Henrik¹  Bock, Elisabeth¹  Andersson, Anna-Maria¹ 

[1] Research Center of Medical Biotechnology, The Protein Laboratory, University of Copenhagen, Panum institute, Blegdamsvej 3C, Building 6.2, DK 2200 Copenhagen, Denmark

关键词: L1;    Adhesion molecule;    Northern blotting;    Brain;    Rat;   

DOI  :  10.1016/0014-5793(91)81350-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Only one copy of the cell adhesion molecule L1 gene is present in the mouse genome, and only one mRNA of 6 kilobases (kb) is expressed in mouse brain [1987, Neurosci. Lett. 82, 89–94]. We have constructed 5 synthetic oligonucleotide probes covering different parts of the published mouse L1 cDNA sequence. Using these probes 3 distinct mRNAs of 9.0, 7.0 and 6.0 kb in rat brain could be demonstrated. Hybridizations performed at different stringency conditions indicated that the 9.0 and 7.0 kb mRNAs were highly related to the L1 mRNA of 6.0 kb expressed in rat brain. The 7.0 kb mRNA is possibly coding for a rat homologue of chicken Nr-CAM, whereas the 9.0 kb mRNA may code for a new member of the L1 family.

【 授权许可】

Unknown