Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes

FEBS Letters

Dahl, Britta¹  Giladi, Eliezer¹  Gaardsvoll, Henrik¹  Bock, Elisabeth¹  Andersson, Anna-Maria¹ 

[1] Research Center of Medical Biotechnology, The Protein Laboratory, University of Copenhagen,34 Sigurdsgade, DK-2200 Copenhagen N, Denmark

关键词: Neural cell adhesion molecule;    Northern blotting;    Oligonucleotide;   

DOI  :  10.1016/0014-5793(90)81420-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain.

【 授权许可】

Unknown