The presence of two types of prorenin converting enzymes in the mouse submandibular gland

FEBS Letters

Murakami, Kazuo²  Nakayama, Kazuhisa¹  Kim, Won-Sin² 

[1] Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan;Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan

关键词: Prorenin converting enzyme;    Epidermal growth factor-binding protein;    Glandular kallikrein;    Serine protease;    Processing;    Submandibular gland;    SMG;    submandibular gland;    PRECE;    prorenin converting enzyme;    EGF-BP;    epidermal growth factor-binding protein;    CHO;    Chinese hamster ovary;   

DOI  :  10.1016/0014-5793(91)81171-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently demonstrated, by protein and cDNA sequence analyses, that prorenin converting enzyme (PRECE) in the ICR mouse submandibular gland is identical to the epidermal growth factor-binding protein (EGF-BP) type B. the mGK-13 gene product identified in Balb/c mouse. However. in the course of cDNA cloning, we noticed the presence of the other cDNA type highly homologous but not identical to the PRECE cDNA. The sequence of the newly identified cDNA was identical to that of the pSGP-2 cDNA cloned from NMRI mice, which also encodes EGF-BP type B different at 9 out of 261 amino acids from the mGK-13 product. Although this difference has been explained by strain polymorphism, our results indicate that these two proteins are distinct gene products. The product of the newly identified cDNA also had a prorenin converting activity. Thus, the products of both cDNAs identified in previous and present studies are involved in maturation of two bioactive polypeptides, renin and EGF.

【 授权许可】

Unknown