Detection of small GTP‐binding proteins in the outer envelope membrane of pea chloroplasts

FEBS Letters

Sekiguchi, Kazumasa¹  Nagano, Yukio¹  Sasaki, Yukiko¹  Matsuno, Ryuichi¹ 

[1] Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto 606-01, Japan

关键词: Chloroplast outer membrane;    Small GTP-binding protein;    Pea;   

DOI  :  10.1016/0014-5793(91)81166-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We found small GTP-binding proteins in the outer envelope membrane of pea chloroplasts. The proteins in this membrane were separated by SDS-PAGE, transferred to a nitrocellulose filter, and incubated with [α-²³P]GTP. Three GTP-binding proteins with the molecular weight of 24 000 were found. Binding was prevented by 10⁻⁸—10⁻⁷ M GTP or by 10⁻⁷ M guanosine 5′-[γ-thio]triphosphate or GDP; binding was unaffected by 10⁻⁸—10⁻⁶ M ATP. Thermolysin treatment of intact chloroplasts resulted in the loss of GTP-binding activity, suggesting that these proteins were in the cytosolic side of the outer envelope membrane.

【 授权许可】

Unknown