Direct measurement of the equilibrium between glutathione and dithiothreitol by high performance liquid chromatography

FEBS Letters

Chau, Mei-Hing¹  Nelson, Jeffrey W.¹ 

[1] Department of Biochemistry, Louisiana State University, Baton Rouge, LA 70803-1806, USA

关键词: Glutathione;    Dithiothreitol;    Thiol reagent;    Disulfide exchange;    GSH;    reduced glutathione;    GSSG;    oxidized glutathione;    DTT or DTTSH SH;    reduced dithiothreitol;    oxidized dithiothreitol;    TFA;    trifluoroacetic acid;    DTNB;    Ellman's reagent [5′;    5′-dithiobis(2-nitrobenzoic acid);    HPLC;    high performance liquid chromatography;    BPTI;    bovine pancreatic trypsin inhibitor;   

DOI  :  10.1016/0014-5793(91)81305-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The equilibrium constant between reduced glutathione (GSH), oxidized glutathione (GSSG), reduced dithiothreitol (DTT^SH _SH), and oxidized dithiothreitol (DTT_S ^S) has been directly measured by high performance liquid chromatography analysis of equilibrium mixtures. The equilibrium constant at 25°C for the reaction GSSG + DTT^SH _SH ⇌ 2GSH + _S ^S varies from approximately 200 M, below pH 8, to approximately 2800 M, above pH 11. The observed pH dependence is generally consistent with published values of acid dissociation constants of these thiols.

【 授权许可】

Unknown