Interaction of the duplicated segment carried by Clostridium thermocellum cellulases with cellulosome components

FEBS Letters

Dhurjati, Prasad²  Béguin, Pierre¹  Tokatlidis, Kostas¹  Salamitou, Sylvie¹  Aubert, Jean-Paul¹ 

[1] Unite de Physiologie Cellulaire and URA 1300 C.N.R.S., Departement des Biotechnologies, Institut Pasteur, 28, rue du Dr Roux, 75724 Paris Cedex 15, France;Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716 USA

关键词: Duplicated segment;    Cellulosome;    Endoglucanase CElD;    Xylanase XynZ;    Clostridium thermocellum;   

DOI  :  10.1016/0014-5793(91)81279-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The function of the non-catalytic, duplicated segment found in C. thermocellum cellulases was investigated. Rabbit antibodies reacting with the duplicated segment of endoglucanase CelD cross-reacted with a variety of cellulosome components ranging between 50 and 100 kDa. ¹²⁵I-labeled forms of CelD and of xylanase XynZ carrying the duplicated segment bound to a set of cellulosome proteins ranging between 66 and 250 kDa, particularly to the 250 kDa S_L (or S1) subunit. ¹²⁵I-labeled forms of CelD and XynZ devoid of the duplicated segment failed to bind to any cellulosome protein. The duplicated segment appears thus to serve to anchor the various cellulosome subunits to the complex by binding to S_L, which may be a scaffolding element of the cellulosome.

【 授权许可】

Unknown