Nuclear localization of budgerigar fledgling disease virus capsid protein VP2 is conferred by residues 308–317

FEBS Letters

Peter, Reiner¹  Rihs, Hans-Peter²  Hobom, Gerd² 

[1] Max-Planck-Institut für Biophysik, Heinrich-Hoffmann-Str. 7, 6000 Frankfurt 71, Germany;Institut für Mikro- und Molekularbiologie, Justus-Liebig-Universität Gießen, Frankfurter Str. 107, 6300 Gießen, Germany

关键词: Nuclear localization sequence;    β-Galactosidase fusion protein;    Budgerigar fledgling disease virus;   

DOI  :  10.1016/0014-5793(91)81090-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The capsid protein VP2 of budgerigar fledgling disease virus (BFDV) contains two sequences (residues 309–315 and 334–340) which are homologous to the prototypic nuclear localization sequence (NLS) of the simian virus 40 T-antigen. Using recombinant potential NLS-β-galactosidase fusion proteins we identified amino acid residues 308–317 (VPKRKRKLPT) to be the NLS of BFDV capsid proteins VP2 and VP3. Microfluorometry studies show that the BFDV-VP2 signal is considerably more efficient in nuclear transport kinetics, than the NLS of SV40-VP2, corresponding to amino acid residues 317–326 (PNKKKRKLSR).

【 授权许可】

Unknown